did you got some amplification ? How did you do that?
I did a PCR once with primers one at 46 and the other one at 71 (long story, but I couldn't design them better no matter what I did, and I struggled quite a lot). Worked perfectly....but I'm the one with a too complicated PCR program
Primer Annealing Temperatures
Posted 26 August 2012 - 08:09 AM
Posted 26 August 2012 - 03:49 PM
Posted 04 October 2012 - 03:00 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 03 March 2014 - 02:31 PM
I know this isn't at all timely, but Andreea mentioned SB buffer. The same authors (Brody and Kern) later published on LB buffer (Lithium instead of sodium). Now I run my gels for 10 min at 300V in Owl chambers rated to 150V and everything is great. LB definitely out-performs SB. Also a phusion lover, I'm going to try your touchdown protocol. I've become less-enamored with touchdowns over the last couple of years, but I'll entertain your protocol.