did you got some amplification ? How did you do that?
I did a PCR once with primers one at 46 and the other one at 71 (long story, but I couldn't design them better no matter what I did, and I struggled quite a lot). Worked perfectly....but I'm the one with a too complicated PCR program
Primer Annealing Temperatures
Posted 26 August 2012 - 08:09 AM
Posted 26 August 2012 - 03:49 PM
Posted 04 October 2012 - 03:00 AM
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon