my pcr product is larger than expected
Posted 20 August 2012 - 12:24 PM
I'm very new to PCR and sequencing, I'm amplyfing a gene of size 560 pb, using published primers, however my product is always larger, I sequenced my product with the same set of primers and I obtained product of 830 bp.
Posted 20 August 2012 - 01:43 PM
Posted 22 August 2012 - 03:10 PM
You can also check the primers with PrimerBLAST, if you didn't do that already (both genomic and RefSeq mRNA databases) and see if you get something matching your product length.
I never trust anything that can't be doubted.
Posted 23 August 2012 - 07:15 AM
Image copyright: Adrian Koh SF.
Replication of this art is strictly prohibited without express permission of the artist