Does anyone know of a DNA marker (ladder) that can be used for Southern blotting?
Thanks
0
1 reply to this topic
#2
yossi.b
-
- Active Members
-
- 11 posts
member
0
Neutral
Neutral
Posted 24 October 2003 - 02:50 AM
u need to do 2 things:
1. take any DNA marker u want, i use the dna ladder from biolabs and run it in your southern gel.
2. while u marked your probe do another probe from bluescript vector and use it in your southern. because there is similarity between the bluescript vector and the marker (they make the marker from bacteria dna) u will get stained marker. of course if u do soutern for bacteria it will not work. it is easy way to mark a dna ladder and without extra cost.
good luck yossi
1. take any DNA marker u want, i use the dna ladder from biolabs and run it in your southern gel.
2. while u marked your probe do another probe from bluescript vector and use it in your southern. because there is similarity between the bluescript vector and the marker (they make the marker from bacteria dna) u will get stained marker. of course if u do soutern for bacteria it will not work. it is easy way to mark a dna ladder and without extra cost.
good luck yossi
