Creating a stable cell line by transfecting a plasmid without any fluorescence t
Posted 20 August 2012 - 12:19 AM
Posted 20 August 2012 - 02:06 AM
Edited by klenow, 20 August 2012 - 02:06 AM.
Posted 19 September 2012 - 06:46 PM
The plasmid should contain some gen that confers resitance agains an antibiotics (puromycin resistant). If this is not the case, you should clone your cDNA into a more appropiate vector because although you can do stable cell line with your plasmid, you will have to check for expression everytime you want to use the cells (and without a visual marker that means to perform PCR or better Q-PCR if you want to compare levels and/or Western blot)... In my opinion, instead of doing all that stuff before any experiment, I would prefer spend one week and clone the cDNA in another vector...
The plasmid does contain a neo resistance. And for your info, I did not construct this plasmid and we got it from another lab - this is a commonly published plasmid.
Posted 20 September 2012 - 01:18 AM