Posted 23 August 2012 - 03:18 PM
Are those problematic reactions on the same run as the OK ones? Do the problematic reactions have some kind of pattern, same wells/wells on the edge?
I've seen such jagged baselines on plates run multiple times (unused wells) on LC480. We are not sure of the reason, one may be the increased elasticity on a used plate and increased evaporation for that reason.
You seem to have ABI Prism however, so you maybe don't even use plates.
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