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Different forward/reverse Primer concentration

telomere primer concentration

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#1 Denise2910

Denise2910

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Posted 18 August 2012 - 10:28 AM

Hello!

I am working on telomere measurement with real time qPCR and wondered why someone should use different concentrations of forward and reverse primers? There are a lot of publications, some researchers use 9 times more reverse than forward, some 3 times more. On what does this depend on?

Would be great to get some help!


Denise





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