Hello!
I am working on telomere measurement with real time qPCR and wondered why someone should use different concentrations of forward and reverse primers? There are a lot of publications, some researchers use 9 times more reverse than forward, some 3 times more. On what does this depend on?
Would be great to get some help!
Denise
0
Different forward/reverse Primer concentration
Started by Denise2910, Aug 18 2012 10:28 AM
telomere primer concentration
No replies to this topic
Also tagged with one or more of these keywords: telomere, primer concentration
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
|
|
|
||
Protocols and Techniques Forums →
Flow Cytometry →
DNA Index?Started by Guest_DaviQd_* , 11 Jun 2012  dna index, telomere, FISH, RTL and 1 more...
|
|
|
