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Why do we use sponges in the Western Transfer?

western blot sponge

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5 replies to this topic

#1 Raquelina

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Posted 16 August 2012 - 05:14 AM

Hi!
Just as a curiosity!

Why do we use sponges in Western Blot transfer?

Thanks!

Have a nice day!

#2 bithorax

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Posted 16 August 2012 - 09:10 AM

haha, interesting question ;)
I guess to keep your membrane and a gel squeezed together, without it those two wouldn't be tight enough.

#3 bob1

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Posted 16 August 2012 - 12:51 PM

BIthorax is correct.

#4 Inmost sun

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Posted 19 August 2012 - 07:10 AM

for semi-dry blotting, to have a stock of transfer buffer; after more than 2h semi-dry blotting, buffer is usually vaporized, without sponges, blotting time should be shorter;

another function is to spread the pressure on the gel more equally...

Edited by Inmost sun, 19 August 2012 - 07:13 AM.


#5 Raquelina

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Posted 20 August 2012 - 03:09 AM

Thank you all!
More or less was what we were thinking of. We also checked the paper where first Western was made and they used Scotch Brite sponges! Sadly, they didn't explain why.

Have a nice day!

#6 bob1

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Posted 20 August 2012 - 01:52 PM

Cheap, easily obtainable, and easy to clean/rinse out - also more permeable to the buffer so you don't get a region where the electrolytes run out during transfer.




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