Hi everyone,
I heard somewhere that you can store foetal calf serum at 4 degrees celsius for about a week, is this true? Or should it always be frozen right up until use? I am just wondering so I can figure out how small my aliquots need to be.
Thanks :-)
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#2
Curtis
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Posted 15 August 2012 - 09:40 PM
Keep your aliquots in 15 ml falcon tubes at -20C for long storage, but if you want to use them in cell culture just take one out and keep in 4C (parafilm it) until you finish it, then take a new one from -20C.
#3
leelee
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Posted 15 August 2012 - 11:25 PM
I freeze my aliquots into single use sizes.
In my opinion, a week at 4C is no problem. I've actually had NCS and FBS at 4C for up to two weeks or so. But unopened, I only open them once, don't want to risk contamination.
In my opinion, a week at 4C is no problem. I've actually had NCS and FBS at 4C for up to two weeks or so. But unopened, I only open them once, don't want to risk contamination.
