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Non-hazardous substitute for ethidium bromide?

non-hazardous DNA stain PCR

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17 replies to this topic

#16 Falco79AD

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Posted 12 September 2012 - 06:53 PM

Here are 2 gel photos. One gel has EtBr in it and the photo was taken on UV light box set at 302 nm. The other gel does not have any dye in it. Instead the DNA was loaded using 6x loading buffer containing 1/100 EZ-Vision stain. This gel photo was taken on UV light box set to 365 nm.

The 6x loading buffer consists of 1x TAE (pH 8.3), 50% sucrose w/v, 0.1% xylene cyanole and 0.1% bromophenol blue.
Add 10 uL of EZ-Vision concentrate (Amresco cat# N391-5MLDRP) to 1 mL of the 6x loading buffer. Keep in dark (use amber tubes). Remember to also use this for your size markers.

Attached Thumbnails

  • 12-Aug 20 EZ-Vision in LB 6 sec NO TOOLS FILTER.jpg
  • 12-Aug 15 EtBr gel 1 sec.jpg


#17 hvripara

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Posted 25 December 2012 - 06:57 PM

you mentioned "We use EZ-Vision concentrate (supplied as 5 mL dropper bottle)"
I did not find the product like you described above in the amresco website, could you tell me the cat number?
thanks,

#18 mdfenko

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Posted 26 December 2012 - 07:57 PM

you mentioned "We use EZ-Vision concentrate (supplied as 5 mL dropper bottle)"
I did not find the product like you described above in the amresco website, could you tell me the cat number?
thanks,

just look at the post immediately before yours
talent does what it can
genius does what it must
i do what i get paid to do





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