Warning: mb_substr_count() expects at most 3 parameters, 4 given in /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php on line 1269

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 110

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 127

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 136

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 137

Warning: Cannot modify header information - headers already sent by (output started at /home/protocol/public_html/forums/admin/sources/classes/text/parser/bbcode/defaults.php:1269) in /home/protocol/public_html/forums/admin/sources/classes/output/formats/html/htmlOutput.php on line 141
coating cover-slides before seeding cells for fluorescent microscopy - Immunology - BioForum

Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

coating cover-slides before seeding cells for fluorescent microscopy


  • Please log in to reply
4 replies to this topic

#1 bithorax

bithorax

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 15 August 2012 - 12:58 PM

does anyone have some experiences with this?
I just need my cells to stick better to the glass, nothing fancy. Im wondering with coating agent to use. Some people use collagen, some with poly L-lysine or some other stuff.
thx in advance!

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,184 posts
481
Excellent

Posted 15 August 2012 - 04:51 PM

It depends on the cell type as to what works best. Collagen (gelatine) and lysine are common ones and are pretty simple to use: make a solution, pour over the coverslips, aspirate and let dry.

#3 bithorax

bithorax

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 16 August 2012 - 08:30 AM

I have found:
http://biosciencetoo...lassCoating.htm :)

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,184 posts
481
Excellent

Posted 16 August 2012 - 12:54 PM

Those look fine. There are many variations on the procedure, depending on who developed it, and people's preferences in terms of work flow. All of them work in my experience.

#5 bithorax

bithorax

    member

  • Active Members
  • Pip
  • 14 posts
0
Neutral

Posted 17 August 2012 - 08:19 AM

Thx! Now it is time for me to get some experience ;)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.