does anyone have some experiences with this?
I just need my cells to stick better to the glass, nothing fancy. Im wondering with coating agent to use. Some people use collagen, some with poly L-lysine or some other stuff.
thx in advance!
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coating cover-slides before seeding cells for fluorescent microscopy
Started by bithorax, Aug 15 2012 12:58 PM
4 replies to this topic
#2
bob1
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Posted 15 August 2012 - 04:51 PM
It depends on the cell type as to what works best. Collagen (gelatine) and lysine are common ones and are pretty simple to use: make a solution, pour over the coverslips, aspirate and let dry.
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bithorax
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#4
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Posted 16 August 2012 - 12:54 PM
Those look fine. There are many variations on the procedure, depending on who developed it, and people's preferences in terms of work flow. All of them work in my experience.
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Posted 17 August 2012 - 08:19 AM
Thx! Now it is time for me to get some experience 
