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coating cover-slides before seeding cells for fluorescent microscopy


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4 replies to this topic

#1 bithorax

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Posted 15 August 2012 - 12:58 PM

does anyone have some experiences with this?
I just need my cells to stick better to the glass, nothing fancy. Im wondering with coating agent to use. Some people use collagen, some with poly L-lysine or some other stuff.
thx in advance!

#2 bob1

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Posted 15 August 2012 - 04:51 PM

It depends on the cell type as to what works best. Collagen (gelatine) and lysine are common ones and are pretty simple to use: make a solution, pour over the coverslips, aspirate and let dry.

#3 bithorax

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Posted 16 August 2012 - 08:30 AM

I have found:
http://biosciencetoo...lassCoating.htm :)

#4 bob1

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Posted 16 August 2012 - 12:54 PM

Those look fine. There are many variations on the procedure, depending on who developed it, and people's preferences in terms of work flow. All of them work in my experience.

#5 bithorax

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Posted 17 August 2012 - 08:19 AM

Thx! Now it is time for me to get some experience ;)




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