I don't have any experience in developing an orthotopic mouse model but will have to work with one soon.
I have a few queries and any help/advice would be much appreciated.
I have to develop a colorectal cancer orthotopic mouse model. I understand there are two ways.
A. inject cell suspension into the cecal wall and
B. implant pieces of subcutaneous tumor into the cecum. I've read that injection of cell suspension does not result in metastasis. Anyways, how do I check which mouse models are compatible with which cell line. Also are nude mice used for xenograft implanted orthotopic models? BALB c or BALB c nu/nu mice ?
Edited by SF_HK, 15 August 2012 - 02:02 AM.