adjusting pH of 4x stacking buffer
Posted 14 August 2012 - 11:36 AM
Posted 14 August 2012 - 01:13 PM
The usual way to do this sort of thing is to make a tris solution of pH 6.8 and add that to your stacking buffer.
Posted 15 August 2012 - 05:07 AM
if you overshoot then you should add more basic component (tris), calculated for a greater volume, readjust pH, then bring to the new final volume.
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Posted 16 August 2012 - 11:16 AM