Crystal violet can bind DNA and can also differenciate adherent cell vs non adherent cell. What the link between adherence and binding DNA???Thanks a lot!
Edited by fabipp, 14 August 2012 - 12:20 AM.
Posted 14 August 2012 - 12:12 AM
Edited by fabipp, 14 August 2012 - 12:20 AM.
Posted 14 August 2012 - 12:39 AM
Posted 14 August 2012 - 12:49 AM
Posted 14 August 2012 - 01:03 PM
Posted 15 August 2012 - 05:04 AM
Posted 15 August 2012 - 12:23 PM
Posted 08 September 2017 - 12:35 AM
Viability cells in microplate using crystal violet
Cell culture 12 wells plates containing colonies, had to be gently washed with PBS and fixed with 3.7% formaldehyde for 10 minutes. Wells are rinsed once again with PBS and colonies are stained with 0.2% crystal violet solution in 10% ethanol for 10 minutes. Excess stain is removed by washing repeatedly with PBS. All the procedures has to be done at room temperature. The plates can be stored at room temperature or at +4 °C for several months without any visible fading of the dye. The crystal violet staining of cells from each well is solubilized using 1 ml of 10% acetic acid and the Abs of the solution is measured at a wavelength of 590 nm.
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