I hope someone can help me.
I'm doing total RNA isolation from brain dead҆s whole blood sample using QIAgen RNA Blood Mini kit. My problem is about agarose gel electrophoresis(2%) of isolated RNA;
- I see an extra band above 18s and 28s rRNAs which is similar in all of my samples.
- After DNase treatment of RNA prior to cDNA synthesis, I can not see any rRNA band in agarose gel electrophoresis! Is it OK?