Posted 13 August 2012 - 02:33 AM
I have one question, i kept my pcr mix including my primer ,template with nuclease free water in the freezer at -20, and will amplify next day.. will it work???? or i have done a mistake icubating it, as taqmaster mix got finished and i have ordered it to make one more pcr.. kindly tell me i am in great tension here..
Posted 13 August 2012 - 03:36 AM
Posted 13 August 2012 - 05:19 AM
Posted 13 August 2012 - 05:22 AM
Posted 13 August 2012 - 05:38 AM
Posted 13 August 2012 - 07:41 AM
In the end of the day: it can work (most probably, if you did not do a big mistake the first time) or it will not work. You will know it only if you try. Otherwise, we can just talk whatifs until the end of time. I always say: while we talked here, you could have tried it already:)