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any tips for doing Fluorescence Western Blot?


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#1 rock&science

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Posted 12 August 2012 - 07:32 PM

Hi guys, our lab decided to change our Western blot approach to fluorescence western blotting. Any tips for this newbie? Thanks

#2 bob1

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Posted 13 August 2012 - 12:49 PM

Assuming that these are the far-red/infra-red based detection systems, there shouldn't be anything different to what you do normally, just follow the recommended antibody dilutions on the data sheets for testing them out.

#3 rock&science

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Posted 13 August 2012 - 04:54 PM

thanks. I just feel nervous trying a new technique. figured I should ask here for tips.




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