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Store western samples in NP-40 lysis buffer?

NP-40 NP40 Western

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#1 Druss00

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Posted 10 August 2012 - 09:09 PM

Hello,

I was just curious if I could store my western samples in NP-40 lysis buffer? Usually I just add loading dye and Beta - Mercap and boil it, but I am going to be short on time tomorrow and was wondering if I could just freeze it down and add the reducing reagent later?

Thanks,
Wes

#2 bob1

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Posted 11 August 2012 - 08:36 PM

Yes, -20 will be fine for a few weeks, longer than that and -80 is best. You can add the reducing agent and loading buffer and boil then freeze if you prefer.





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