1 reply to this topic

[Druss00]

Hello,

I was just curious if I could store my western samples in NP-40 lysis buffer?  Usually I just add loading dye and Beta - Mercap and boil it, but I am going to be short on time tomorrow and was wondering if I could just freeze it down and add the reducing reagent later?

Thanks,
Wes

[bob1]

Yes, -20 will be fine for a few weeks, longer than that and -80 is best.  You can add the reducing agent and loading buffer and boil then freeze if you prefer.