I got a free sample of Q5 polymerase from NEB, and tested it out. Worked first time with remarkable processivity, copying a 2 kb region with a 30 second extension. I used the hot start version. This looks to be as good or better than Phusion.
0
1 reply to this topic
#2
Curtis
-
- Global Moderators
-
- 965 posts
Metaller Scientist
42
Excellent
Excellent
Posted 10 August 2012 - 07:51 PM
interesting. i wish there were rt enzymes that could synthesize long cdna too, so that i didn't have to amplify small fragments to fuse them together with phusion. the problem with phusion is its higher tm compared to other products, making it not really suitable for oe-pcr
Also tagged with one or more of these keywords: PCR, NEB
 |
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
High fidelity PCR trouble shootingStarted by Guest_uday_* , Yesterday, 06:59 PM  high fidelity, pcr
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Reproducible Non-Specific PCR ProductStarted by Guest_Epigeneticist_* , 20 May 2013 PCR, Sequencing, Cloning
|
|
|
|
|
|
Protocols and Techniques Forums →
Tissue and Cell Culture →
Housekeeper for HEK cells?Started by Guest_Stephp_* , 17 May 2013 HEK cells, house keeper, PCR and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Cloning large fragmentsStarted by Guest_bknm_* , 15 May 2013 Cloning, PCR, large inserts
|
|
|
|
|
|
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Can't get PCR with large overhang primers to workStarted by Guest_EtOH_* , 10 May 2013 PCR, overhang, low gc
|
|
|
