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Question on fundamental 2D gel concept - identical amount of proteins loaded


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#1 barnacleman

barnacleman

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Posted 09 August 2012 - 05:39 PM

Hi,

For 2D gels, an identical amount (e.g. 200 microgram) of proteins were loaded onto each gel.

As such, how can we see the different protein expression level or different spot showing up?

Is it that:
(1) There are both simultaneous up- as well as down-regulation. Somehow they balance out each other?
(2) How about in cases of metabolic suppression, whereby there is a general global decrease in protein expression. If we load an identical amount of proteins, what do you expect to see in the 2D gels?
(3) They are changes in protein expression outside the 2D gel resolving range (pI and MW range)?

Please shed some light on this
Thank you in advance!

Edited by barnacleman, 09 August 2012 - 05:41 PM.





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