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Injecting mice with lentivirus

mice lentivirus

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#1 Johndg2

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Posted 09 August 2012 - 01:09 PM

Hello all,
I am new to lentiviral production and I'm in the middle of writting my proposal. I need to clone my GOI into a lentivirus, transfect them into 293 cells, test for expression and, then inject them into mice. I'm studying adipogenesis as it relates to obesity, so can I just inject my clone into subcutaneous fat of mice? Any help is greatly appreciated!

#2 bob1

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Posted 10 August 2012 - 12:49 AM

Potentially... don't forget your ethics approvals beore trying this. You may want to test the system using adipocyte cultures first.





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