Does anyone know a way to remove excess ethanol from DNA dissolved in about 50ul of 1/5th TE buffer? I didn't fully dry the ETOH from a few samples and I am afraid it will cause problems downstream.
Submit your paper to J Biol Methods today!
ethanol in final DNA sample
1 reply to this topic
Posted 09 August 2012 - 11:48 AM
The most foolproof way would be to reprecipitate your DNA. Add 1/10 volume of 3M sodium acetate, pH 5.2, add 2.5 volumes ethanol, spin to precipitate, and then wash with 70% ethanol, spin, and dry well this time. You could also speedvac all of the liquid and redissolve, but the pellet might be hard to dissolve.