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ethanol in final DNA sample


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#1 microbe hunter

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Posted 09 August 2012 - 11:35 AM

Does anyone know a way to remove excess ethanol from DNA dissolved in about 50ul of 1/5th TE buffer? I didn't fully dry the ETOH from a few samples and I am afraid it will cause problems downstream.

#2 phage434

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Posted 09 August 2012 - 11:48 AM

The most foolproof way would be to reprecipitate your DNA. Add 1/10 volume of 3M sodium acetate, pH 5.2, add 2.5 volumes ethanol, spin to precipitate, and then wash with 70% ethanol, spin, and dry well this time. You could also speedvac all of the liquid and redissolve, but the pellet might be hard to dissolve.




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