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[Question] How to remove cell debris stuck on the attached cells in primary card

primary culture cardiomyocyes

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#1 lydialing

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Posted 09 August 2012 - 05:28 AM

Dear all,

I encounter a problem of clung cells/debris while doing rat cardiomyocyte culture. After 2 days of seeding, using DMEM, I tap the dish hard to wash away tissue/cell debris. However, even after more than 5 times of wash, I can still find some cell clumps are very sticky and difficult to remove. Clinging on the beating cardiomyocytes and piling up high, these clumps cause a great trouble for me to take clear images. A picture is attached for your reference.
Is there any way to remove these masses and leave the seeded and attached cardiomyocytes not affected? Thank you very much.

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  • A_Ch 2_042.JPG


#2 leelee

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Posted 09 August 2012 - 07:18 PM

You could use a P200 and suck the clumps away individually :)

#3 lydialing

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Posted 11 August 2012 - 07:42 AM

You could use a P200 and suck the clumps away individually Posted Image

In addition to the larger clump, many are singles cells that instead of adhere to the well but landed on other cells and stick there. They can be seen only under a microscope.





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