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Changes to housekeeping loading controls after drug treatment


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#1 science noob

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Posted 08 August 2012 - 08:19 PM

Treatment of a particular drug on my cell line seem to affect cell morphology and levels of tubulin and actin. This voids the use of these loading controls for reference in a western blot to my marker of interest. Are there any other housekeeping proteins which are abundantly expressed in all cells that I could use?

#2 gyma

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Posted 09 August 2012 - 01:34 AM

at least you can try GAPDH. also you can use real time pcr as an alternative way. in that case, 18s rRNA is also suitable.

#3 bob1

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Posted 09 August 2012 - 01:26 PM

Histone, and nucleolin also work. You can also measure the concentration of protein in your samples and load relative to that.




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