Hi
I'm trying to induce rat mesenchymal stem cells to differentiate into adipocytes. The protocol I have in hand mentions indomethacin in the DMEM medium. However, indomethacin powder is the only thing I have and it does not dissolve in DMEM nor PBS. any ideas?
Thanks!
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Powder indomethacin in cell culture media??
Started by The Dark Knight Pipettes, Aug 08 2012 03:14 PM
indomethacin DMEM cell culture
2 replies to this topic
#2
bob1
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Posted 08 August 2012 - 06:23 PM
Try DMSO or DMF (di methyl formamide). The product sheet should tell you the solubility in different solutions.
#3
The Dark Knight Pipettes
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Posted 09 August 2012 - 01:04 PM
DMSO works like a charm. Thanks Bob.
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