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Powder indomethacin in cell culture media??

indomethacin DMEM cell culture

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#1 The Dark Knight Pipettes

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Posted 08 August 2012 - 03:14 PM

Hi

I'm trying to induce rat mesenchymal stem cells to differentiate into adipocytes. The protocol I have in hand mentions indomethacin in the DMEM medium. However, indomethacin powder is the only thing I have and it does not dissolve in DMEM nor PBS. any ideas?

Thanks!

#2 bob1

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Posted 08 August 2012 - 06:23 PM

Try DMSO or DMF (di methyl formamide). The product sheet should tell you the solubility in different solutions.

#3 The Dark Knight Pipettes

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Posted 09 August 2012 - 01:04 PM

DMSO works like a charm. Thanks Bob.





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