1. Can I just follow protocols online to make my own chemically competent cells? Besides the additional plasmid for rare tRNA, these cells are not much different from other competent cells, right?
2. I read the transformation protocol provided by the Strategene, there are two specific things required for the transformation. The XL10-Gold
β-mercaptoethanol and 14-ml BD Falcon Polypropylene Round-Bottom Tubes. They say the efficiency is not guaranteed if using other 2-ME or other tubes. Has anyone used just normal 2-ME or other shapes of polypropylene tubes before? Maybe can share how long the heat pulse should be? I asked this because, if I want to make my own cells, and I don't have the XL10-Gold 2-ME (they don't sell it separately, either). Also, 14ml BD Falcon tubes are not cheap.
Edited by sl17, 08 August 2012 - 12:27 PM.