"pinned its limbs on sponge board "
No one in there right mind is going to give you ethics approval for such a cruel procedure! That poor mouse will have numerous injuries from being pinned out each week. What kind of person even suggests sticking pins in a live mouse!!! Can you imagine waking up from anaesthetic with big ol' pin holes in your arms?
Okay, personal outrage aside, here is what I would do:
- using an inhaled anaesthetic (e.g. methoxyfluorane), wait until the mouse appears drowsy and has stopped wondering around the jar, but isn't fully under
- take her out, and gently (using blunt forceps) pull her tongue out and have a look, you'll have to be really careful to hold her tongue lightly so you don't hurt or bruise it
If you are holding her correctly, you should have no trouble with this. If you are using a particularly feisty mouse strain, you may need to knock the mouse completely out, but even so if you go easy on that anaesthetic they will recover well.
I use this method to take saliva samples from mice, every two days. They appear to tolerate the anaesthetic well (in fact I wonder if some take it too well as they seem less and less fussed about getting in the jar each time, little addicts!).
Edited by leelee, 08 August 2012 - 01:59 AM.