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Bacteria grow on MSM agar plate without carbon source

MSM agar noble

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#1 kimoe



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Posted 06 August 2012 - 08:00 PM

Hi all,

Recently I conducted experiment on growing bacteria on MSM agar plate with various C-source (using the spot test). To my surprise, I found bacteria grew on the control plate without C-source. It only happened to two bacteria genus (I tested six).
Is it really possible for certain bacteria to eat the agar as their carbon source? (I used agar noble)

#2 El Crazy Xabi

El Crazy Xabi


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Posted 20 August 2012 - 04:41 PM

Do you mean the medium described here?

Minimal salts medium for studies of hydrocarbon degradation in soil contained 4 g of NaNO3, 1.5 g of KH2PO4, 0.5 g of Na2HPO4, 0.0011 g of FeSO4 · 7H2O, 0.2 g of MgSO4 · 7H2O, and 0.01 g of CaCl2 per liter of water at pH 7.0

Yes, there are microorganisms capable of using agar as C-source. Also could be some C-fixation if there is a suitable E-source.

Do you wash your inocula? Residual media may be present. In this case, colonies are usually punctiform.

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