Can ELISA standards be used as antigens?Monoclonal Ab
Posted 06 August 2012 - 01:59 AM
As a first part of making a mouse monoclonal antibody against a murine cytokine by hybridoma cells, I was wondering:
Can an ELISA standard like mouse IFN-gamma (found in a commercial ELISA kit), mixed with an adjuvant (e.g. TiterMax) be injected to mice in order to induce the B-lymphocytes to generate anti-IFN-gamma antibody?
Does this sound theoretically as a plausible approach?
With many thanks,
Posted 06 August 2012 - 08:13 AM
Posted 16 October 2012 - 01:27 AM
Simply buy the recombinant protein iteself.