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Can ELISA standards be used as antigens?

Monoclonal Ab

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3 replies to this topic

#1 IPI

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Posted 06 August 2012 - 01:59 AM

Hi There,

As a first part of making a mouse monoclonal antibody against a murine cytokine by hybridoma cells, I was wondering:

Can an ELISA standard like mouse IFN-gamma (found in a commercial ELISA kit), mixed with an adjuvant (e.g. TiterMax) be injected to mice in order to induce the B-lymphocytes to generate anti-IFN-gamma antibody?

Does this sound theoretically as a plausible approach?

With many thanks,

#2 Astarte Biologics

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Posted 06 August 2012 - 08:13 AM

Sure its plausible. Since monoclonals bind to the standards clearly the standards are antigenic. Since it is an endogenous protein and relatively small it might be beneficial to conjugate it to something like KLH to stimulate a strong response.

Good luck

#3 IPI

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Posted 06 August 2012 - 09:03 PM

Many thanks for your response Astarte Biologics.

#4 wernerlael

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Posted 16 October 2012 - 01:27 AM

I agree that it can be done. However, in my opinion, this will not be acceptable for good journals.
Simply buy the recombinant protein iteself.





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