Enzyme storage in eppendorphs to use in enzyme assay next day
Posted 05 August 2012 - 10:06 AM
I have another question too. I am working on Cellulases from Bacteria. Is it possible that i centrifuge the culture medium in eppendorphs to get the crude enzyme but use this crude enzyme next day to perform assay for different parameters in its charaterization? If i keep this crude enzyme in refrigerator for a night, will it change the nature of the enzyme?
Posted 05 August 2012 - 01:52 PM
Posted 06 August 2012 - 11:52 AM
I actually, want to save my time by acquiring crude enzyme by producing it on optimised conditions once for all, for the use in characterization and later on for purification by Ammonium sulphate precipitation method.
Posted 06 August 2012 - 04:43 PM
Posted 07 August 2012 - 10:53 AM
And how can i know if this is a stable enzyme or not? by performing assays?