Conversion of Absorbance into enzyme units
Posted 05 August 2012 - 06:49 AM
I'm working on cellulases from bacteria. I've taken O.D after several parameters to check the enzyme activity. I'm using Sodium Acetate buffer of pH 5 (0.1 M) in my enzyme assay. I take 0.2 ml crude enzyme, 0.5 ml substrate buffer (substrate prepared in Acetate buffer of pH 5 (0.1M)) ad 0.3 ml of Acetate buffer as stated above. It makes up the reaction mixture 1 ml in total. I incubate it at 50 C for 15 mins, then add 3 ml DNS and then boil for 5 mins, cool down and take the Absorbance. For example, if Spec give the reading as 0.375. How would i convert it into enzyme units?
I have tried to search it on internet but couldnt find anything understandable.
Thanks in advance
Posted 06 August 2012 - 11:44 AM
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Posted 07 August 2012 - 10:55 AM
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Posted 09 August 2012 - 09:15 AM