small dots in cell culture
Posted 04 August 2012 - 02:17 PM
I work on PC-3 and NIH-3T3 cell lines. It is for 2 weeks that I have small dots in my culture that slow the rate of cell growth. they appear after the first passage of defreezed cells but in free culture medium they don't appear. Thses dots result in cell death and collapse. has every body any helpful idea?
Posted 04 August 2012 - 02:41 PM
Posted 04 August 2012 - 02:43 PM
Read the thread on black dots in cell culture, which is pinned at the top of the cell culture subforum. Like pcrman suggested while I was typing