Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How delete survival gene in bacteria still keeping cell alive.

Survival gene Gene knockout

  • Please log in to reply
5 replies to this topic

#1 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
21
Excellent

Posted 02 August 2012 - 09:35 PM

How delete survival gene in bacteria still keeping cell alive. How we can know given gene is essential for survival ?

#2 leelee

leelee

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 652 posts
53
Excellent

Posted 02 August 2012 - 10:44 PM

Put simply, you can't. If a gene is essential to survival, then the bacteria simply can't survive without it.

(unless, in some cases, you want to supplement that gene product from elsewhere, e.g. a plasmid or in the media itself).

#3 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
21
Excellent

Posted 03 August 2012 - 06:21 AM

Thanks for reply. How one is supposed to know that given gene is essential for survival? trying deleting gene probably will not give colony. what if survival gene product could not be supplied from outside media.

#4 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,327 posts
80
Excellent

Posted 03 August 2012 - 07:12 AM

Thanks for reply. How one is supposed to know that given gene is essential for survival? trying deleting gene probably will not give colony. what if survival gene product could not be supplied from outside media.

Well if you delete 1 gene and the bacteria dont survive ... then you kno its a gene essential for survival.

And what do you mean by "what is survival gene product could not be supplied from outside media" ?
Are you talking about "food" that bacteria need to survive ?

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#5 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
21
Excellent

Posted 03 August 2012 - 07:28 AM

How can we select bacterial mutant on growth medium if survival gene is deleted. merely no colony to judge as gene is essential for growth may not be effective. Reason for no colony might be errors in experiment or homologous recombination problem.

what if survival product is not food but some internal machinery of bacteria say DNA polymerase which could not be supplied from outside.

#6 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,327 posts
80
Excellent

Posted 03 August 2012 - 01:01 PM

How can we select bacterial mutant on growth medium if survival gene is deleted. merely no colony to judge as gene is essential for growth may not be effective. Reason for no colony might be errors in experiment or homologous recombination problem.

what if survival product is not food but some internal machinery of bacteria say DNA polymerase which could not be supplied from outside.

You are right.

The problem with deleting or mutating an essential gene does indeed imply that the bacterium wont grow...
See the attachement on how researchers look for essential genes.
And check this: http://www.nature.co...p00125.html#/f2, check figure 1!

Also: to make sure its not because of the medium, you use the same medium as when the gene was not distrupted.

And about the internal machinery of bacteria not be supplied from outside: not sure what you mean by this... If it can not be supplied from outside when you altered the gene.. it will also not be supplied from outside without altering the gene (unless its a gene for a transporter that brings in , for example, a certain nutrient... but then again: it means that the gene you changed is essential for survival).

Attached Files


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.






Also tagged with one or more of these keywords: Survival gene, Gene knockout

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.