Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Electroporation of J774.2 murine macrophage cell line

  • Please log in to reply
1 reply to this topic

#1 mirr



  • Members
  • Pip
  • 1 posts

Posted 02 August 2012 - 05:25 AM


Does anyone have experience with transfection (electroporation) of the murine macrophage cell line J774.2?

I am planning to use the Neon Transfection System (Invitrogen), and I would be interested in knowing what electroporation parameters to use. I know I can always run an optimization protocol and identify optimal parameters, but maybe someone out there has some experience on this matter already..?

Best regards Mie

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,504 posts

Posted 02 August 2012 - 01:24 PM

I would have a look on the invitrogen website for the neon system - there are a lot of cell lines' conditions listed on there (but not J774.2, though they do have "macrophage listed). You may be able to come up with a consensus on the conditions that will be fairly close to optimal.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.