Does anyone have experience with transfection (electroporation) of the murine macrophage cell line J774.2?
I am planning to use the Neon Transfection System (Invitrogen), and I would be interested in knowing what electroporation parameters to use. I know I can always run an optimization protocol and identify optimal parameters, but maybe someone out there has some experience on this matter already..?
Best regards Mie
Electroporation of J774.2 murine macrophage cell line
1 reply to this topic
Posted 02 August 2012 - 01:24 PM
I would have a look on the invitrogen website for the neon system - there are a lot of cell lines' conditions listed on there (but not J774.2, though they do have "macrophage listed). You may be able to come up with a consensus on the conditions that will be fairly close to optimal.