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Alternative to gene silencing for functional assay?? much help needed :)

shRNA

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#1 Octavina Sukarta

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Posted 02 August 2012 - 04:06 AM

Hi,
I have question regarding functional analysis. I basically have a plant receptor and we'd like to know whether its activity is dependant on a co-receptor in the same plant. To do this our main strategy was to perform transient gene silencing of the co-receptor by siRNA (using hairpin RNA). But unfortunately, the qPCR analysis consistently shows that the co-receptor gene is not being silenced Posted Image ..

Is there an alternative technique that I can use to show that the receptor is dependant on this co-receptor for activity other than silencing?

Edited by Octavina Sukarta, 02 August 2012 - 04:08 AM.


#2 bob1

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Posted 04 August 2012 - 02:52 PM

Is there any potential for using a drug that might target the co-receptor? Do you think the interaction is direct? If so, you may be able to do something like a co-immunoprecipitation. Reporter gene assay for a particular down stream target?





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