Hi,
In previous publications I read when somebody is trying to characterize a protein they measure pI of the protein. Any idea why this pI value is important in biological aspect?
Thanks.
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#2
pDNA
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Posted 02 August 2012 - 11:04 AM
E.g. a protein can be precipitated at its pI.
Regards,
p
Regards,
p
#3
sb21
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Posted 03 August 2012 - 02:59 AM
Hi pDNA,
Thanks for the reply. In my lab we usually perform co-IP to precipitate the protein as in this method we do not destroy the other proteins linked with my interest of protein. do you think protein precipitation at its pI will be helpul any other way?
Thanks for the reply. In my lab we usually perform co-IP to precipitate the protein as in this method we do not destroy the other proteins linked with my interest of protein. do you think protein precipitation at its pI will be helpul any other way?
#4
mdfenko
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Posted 03 August 2012 - 06:43 AM
you can use isoelectric precipitation (precipitation at the pI of the protein) as a purification step.
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genius does what it must
i do what i get paid to do
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