Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Determining pI of a protein

pI

  • Please log in to reply
3 replies to this topic

#1 sb21

sb21

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 02 August 2012 - 03:22 AM

Hi,

In previous publications I read when somebody is trying to characterize a protein they measure pI of the protein. Any idea why this pI value is important in biological aspect?

Thanks.

#2 pDNA

pDNA

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 493 posts
14
Good

Posted 02 August 2012 - 11:04 AM

E.g. a protein can be precipitated at its pI.

Regards,
p

#3 sb21

sb21

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 03 August 2012 - 02:59 AM

Hi pDNA,

Thanks for the reply. In my lab we usually perform co-IP to precipitate the protein as in this method we do not destroy the other proteins linked with my interest of protein. do you think protein precipitation at its pI will be helpul any other way?

#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,785 posts
132
Excellent

Posted 03 August 2012 - 06:43 AM

you can use isoelectric precipitation (precipitation at the pI of the protein) as a purification step.
talent does what it can
genius does what it must
i do what i get paid to do





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.