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mamallian expression vector that has FLAG tag and GFP


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3 replies to this topic

#1 rock&science

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Posted 01 August 2012 - 04:56 PM

Hi,

Just wondering whether anyone aware of an expression vector that has C-terminal FLAG-tag and GFP at the same time? I have been googling with no luck. Thanks

#2 bob1

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Posted 01 August 2012 - 05:45 PM

I'm not aware of any, but you should be able to add a flag tag by PCR if necessary. I don't really see the reason to have two tags on the same end of the protein though...

#3 rock&science

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Posted 01 August 2012 - 05:56 PM

thanks for the response. the reason is that my protein is n-terminally activated, so I need a tag on C-term for purification, whereas GFP is for tracking purposes.

Can you recommend a good GFP expression vector?

Edited by rock&science, 01 August 2012 - 05:57 PM.


#4 Curtis

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Posted 01 August 2012 - 08:50 PM

phMGFP from promega, and pEGFPN2 from clontech. They must have newer version too.

keep in mind that having two tags will increase the size of your plasmid.




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