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storing PCR product overnight

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2 replies to this topic

#1 fishtricks-x



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Posted 01 August 2012 - 08:48 AM

is it allowable for PCR product to store overnight at 19C?

#2 hobglobin


    Growing old is mandatory, growing up is optional...

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Posted 01 August 2012 - 10:17 AM

I'd avoid it if possible...10 degrees is maximum for my samples

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that did belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 bob1


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Posted 01 August 2012 - 01:38 PM

It depends on what you want to do with the product and the polymerase used to generate it - if it is a Taq (or other non-proofreading) based PCR, it will probably be fine Any proofreadin polymerase will nibble away the ends, so if you want to clone or sequence the PCR or something similar, it won't be any good.

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