is it allowable for PCR product to store overnight at 19C?
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#2
hobglobin
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Posted 01 August 2012 - 10:17 AM
I'd avoid it if possible...10 degrees is maximum for my samples
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#3
bob1
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Thelymitra pulchella
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Posted 01 August 2012 - 01:38 PM
It depends on what you want to do with the product and the polymerase used to generate it - if it is a Taq (or other non-proofreading) based PCR, it will probably be fine Any proofreadin polymerase will nibble away the ends, so if you want to clone or sequence the PCR or something similar, it won't be any good.
