Clean genomic DNA and plasmid DNA extraction from E.coli
Posted 01 August 2012 - 08:33 AM
I am interested in isolating relatively pure genomic DNA and plasmid DNA from E.coli. Are there protocols which would ensure the purity of the extracted samples. e.g. no gDNA in plasmid preps and vice versa.
I am familiar with the standard plasmid prep techniques and how to avoid gDNA contamination. Just wanted to know if there is a method of isolating pure genomic DNA by avoiding plasmid contamination.
Thanks in advance.
Posted 01 August 2012 - 01:42 PM
I never trust anything that can't be doubted.
Posted 10 January 2013 - 03:26 PM
In general when doing a gDNA prep do plasmids get pulled down with it?
Specifically we have a low copy plasmid that we are trying to use for homologous recombination
and we are trying to screen for the first recombination event. I suppose if a cross over has occured
the plasmid backbone will be attached to gDNA. However if recom. has not occured we could still get
non-specific amp if plasmid gets pulled down in the gDNA prep.
Hope I haven't confused
Posted 10 January 2013 - 04:48 PM