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Problems with PAGE gels

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#1 helencollins560



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Posted 01 August 2012 - 07:56 AM

I need to make a 40% acrylamide stock (100g acrylamide/bisacryl (19:1) in 175ml distilled water)

however, we do not have any acrylamide/bisacryl (19:1) in powered form, but we have plenty of biorad 30% acrylamide/bisacryl (29:1) solution is there any way of me being able to make the solution i need using this? if so, how would i go about doing that?

i was thinking i could either purchase some acrylamide/bisacryl (19:1) in powered form or 40% acrylamide/bisacryl (19:1) liquid form

any advice would be appreciated!!!

#2 bob1


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Posted 01 August 2012 - 01:55 PM

You can never make a more concentrated solution from a less concentrated one without evaporating some of the fluid. You will either have to buy in a stock (I really really recommend pre-made acrylamide stocks, so much less hassle), or buy in the powders.

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