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yeast one hybrid screen (y1H) in drosophila

yeast one hybrid screen drosophila

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#1 Rohit Joshi

Rohit Joshi

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Posted 01 August 2012 - 01:54 AM

I am trying to find out the transcription factors that may bind to a 600bp cis-regulatory DNA element of my interest. Therefore I am planning to do a yeast 1-hybrid screen for all the TFs cDNA that may bind to this DNA elements.
I need suggestions to this effect. I will be screening Drosophila cDNA library for this.

What is the best way to do this?

Is there any kit available to do this?

What will be best way to tag cDNAs with GAL4 Activation domain?

Any suggestions will be appreciated
Thanks





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