hi
is there is a differences between mRNA extracted from Plasma and mRNA extracted from whole blood?
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#2
gyma
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Posted 31 July 2012 - 04:46 PM
blood=plasma+cells(wbc, rbc, platelet etc.). what kind of mRNA can you get from plasma since there are no cells at all.
#3
Trof
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Posted 01 August 2012 - 01:54 PM
gyma, on 31 July 2012 - 04:46 PM, said:
blood=plasma+cells(wbc, rbc, platelet etc.). what kind of mRNA can you get from plasma since there are no cells at all.
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Bassam27
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Posted 01 August 2012 - 03:42 PM
thank u for replying . i need to measure the level of expresssion of mRNA OF ERBB2 IN breast cancer and i read article confrm that there is mRNA ERBB2 CELL CIRCULATING IN BLOOD BUT other aricle confirm that they cand detect mRNA OF ERRBB2 IN PLASMA BCZ THAT I AM NOT SURE IF I UES WHOLE BLOOD DIRECTLY TO REACTION IS GOING TO WORK OR NOT
#5
Bassam27
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Posted 01 August 2012 - 03:45 PM
Can i speak to any one who have back ground about housekeeping gene that ues in qRT PCR ?
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Posted 01 August 2012 - 04:21 PM
Bassam27, on 01 August 2012 - 03:45 PM, said:
Can i speak to any one who have back ground about housekeeping gene that ues in qRT PCR ?
