mRNA IN BLOOD
Posted 31 July 2012 - 04:07 PM
is there is a differences between mRNA extracted from Plasma and mRNA extracted from whole blood?
Posted 31 July 2012 - 04:46 PM
Posted 01 August 2012 - 01:54 PM
For example circulating RNA from dead cells used as a cancer biomarker. Also the can be viral RNA and microRNA in plasma.
blood=plasma+cells(wbc, rbc, platelet etc.). what kind of mRNA can you get from plasma since there are no cells at all.
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Posted 01 August 2012 - 03:42 PM
Posted 01 August 2012 - 03:45 PM
Posted 01 August 2012 - 04:21 PM
I have used b-actin and gapdh and they both work well. however, I dont know how can you normalize ERBB2 mRNA. how do you separate correctly the cells you want?
Can i speak to any one who have back ground about housekeeping gene that ues in qRT PCR ?