Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

About trypsinizing adherent cells in 96-well plates


  • Please log in to reply
2 replies to this topic

#1 labbla

labbla

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 31 July 2012 - 10:52 AM

Hello,

I want to trypsinize adherent cells growing in a 96-well plate. I have generally seen people trypsinize cells in bigger flasks so I was wondering if this is a normal thing to do? Should I be following the same procedure as with bigger flasks except lower the volume of trypsin? I really appereciate your time in helping me. :)

Thanks!

#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,101 posts
57
Excellent

Posted 01 August 2012 - 12:45 AM

yes you can, just scale down. take a look at this website, it might help:

http://www.invitroge...bers.html#prot1

But we usually use less than what this page suggests. We add 500 ul - 1 ml of trypsin to T75 flasks. this amount could be different in other labs. for T25 we add 300 ul to 500 ul.

#3 leelee

leelee

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 652 posts
52
Excellent

Posted 01 August 2012 - 04:59 AM

I agree, yes you can. I would probably use 100ul of trypsin per well.

What do you plan to do with the cells after you've lifted them?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.