About trypsinizing adherent cells in 96-well plates
Posted 31 July 2012 - 10:52 AM
I want to trypsinize adherent cells growing in a 96-well plate. I have generally seen people trypsinize cells in bigger flasks so I was wondering if this is a normal thing to do? Should I be following the same procedure as with bigger flasks except lower the volume of trypsin? I really appereciate your time in helping me.
Posted 01 August 2012 - 12:45 AM
But we usually use less than what this page suggests. We add 500 ul - 1 ml of trypsin to T75 flasks. this amount could be different in other labs. for T25 we add 300 ul to 500 ul.
Posted 01 August 2012 - 04:59 AM
What do you plan to do with the cells after you've lifted them?