Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Discrepancies between miRNA Array Data and PCR Data

microRNA affymetrix real time-PCR taqman assays

  • Please log in to reply
1 reply to this topic

#1 jtapocik

jtapocik

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 31 July 2012 - 10:36 AM

I am having opposing confirmations with my taqman miRNA PCR for my array data (the miRNA will be significantly upregulated in the array, and then significantly downregulated when PCR'd). Does anyone else run into this issue? Anyone figure out a way to solve this issue?
Help is greatly appreciated!!! Posted Image

#2 Trof

Trof

    Brain on a stick

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,160 posts
102
Excellent

Posted 01 August 2012 - 08:25 AM

Basically, trust the PCR more.
(of course after your check your primers and assay for specifity and sensitivity or try to design different (but that would be hard I guess for miRNA) primers for the same target to confirm, so that you are completely sure there could't be mistake in your assay)

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.