Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Creating a growth curve for bacteria- reaching various log phases

log phase growth curve bacteria vibrio splendidus

  • Please log in to reply
1 reply to this topic

#1 mbraun

mbraun

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 31 July 2012 - 06:29 AM

Hi! I need to create a growth curve for my sample of Vibrio splendidus. I've done the growth curve a few times and have reached log phase OD600 readings of anywhere between 0.71-1.03, when I'm typically expecting log phase of 0.8ish. I've repeated this experiment about six times and am wondering if the log phase can start to plateau at different OD600 depending on the culture. I've been growing mine samples up in TSB+2%NaCl, but have used differenet batches of it.
Has anyone else experienced this?

#2 El Crazy Xabi

El Crazy Xabi

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 199 posts
20
Excellent

Posted 31 July 2012 - 07:08 PM

Do you use the same exact size of inoculum? number of cells, UFC,...
Does the inoculum come from the same origin/culture medium with the same age, temperature, agitation speed, volume,...?
Do you get the inoculum from the log phase?

The growth curve is dependent on all those parameters





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.