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Recovery of viral RNA

rna recovery rna separation

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3 replies to this topic

#1 dryas

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Posted 31 July 2012 - 06:02 AM

Dear all,

I have to try to isolate a specific viral RNA from total RNA. I'd like to use the method magnetic beads streptavidin and a biotinylated probe to hybridise my viral RNA.
Does someone know a valid protocol?

Thanks

#2 Curtis

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Posted 31 July 2012 - 08:47 PM

this is a very good question. I would also like to know the answer. Which company did you buy it from? Maybe you need to contact the company you bought it from?

#3 dryas

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Posted 31 July 2012 - 10:10 PM

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I haven't yet bought the reagents, and before to do it I'd like to know if someone has some useful suggestions..
For example: company, type of bead (there are more than one), ecc..

#4 Curtis

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Excellent

Posted 31 July 2012 - 10:13 PM

Dynalbeads are famous. check Invitrogen website:

http://www.invitroge...ml?CID=fl-dynal




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