Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

PFA - does it have to be immediately chilled?


  • Please log in to reply
2 replies to this topic

#1 gregg

gregg

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 30 July 2012 - 01:42 PM

Hi all

Currently attempting to climb the vertical learning curve that is EngD year 1 and I was wondering if someone could help. I made some 4% PFA for the first time today, for the purpose of fixing some cells for FISH. The protocol said that after heating the water / PBS and dissolving the paraformaldehyde the solution should be chilled on ice. Is this purely to prevent the PFA "going off" or is it a vital part of the procedure? I ask because I'm stupid and I forgot to immediately chill it, however I did aliquot the PFA and get it in the freezer within 30mins of making it.

Thanks for your advice.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,830 posts
414
Excellent

Posted 30 July 2012 - 05:10 PM

It will be fine, it is stable in the fridge for about a week and well over 6 months at -20 C. Chilled fixatives are often used to slow the cell processes and the rate of action of the fixatives during use.

#3 gregg

gregg

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 30 July 2012 - 11:15 PM

Excellent. Thank you!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.