Hi all
Currently attempting to climb the vertical learning curve that is EngD year 1 and I was wondering if someone could help. I made some 4% PFA for the first time today, for the purpose of fixing some cells for FISH. The protocol said that after heating the water / PBS and dissolving the paraformaldehyde the solution should be chilled on ice. Is this purely to prevent the PFA "going off" or is it a vital part of the procedure? I ask because I'm stupid and I forgot to immediately chill it, however I did aliquot the PFA and get it in the freezer within 30mins of making it.
Thanks for your advice.
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bob1
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Thelymitra pulchella
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Posted 30 July 2012 - 05:10 PM
It will be fine, it is stable in the fridge for about a week and well over 6 months at -20 C. Chilled fixatives are often used to slow the cell processes and the rate of action of the fixatives during use.
