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[Pombe]

I have read several topics from this forum which recommend preparing homemade competent cells in CCMB80 buffer instead of CaCl₂.  This purportedly increases transformation efficiency by several fold or even orders of magnitude.  Most topics link to this protocol, (http://openwetware.o...competent_cells) which has been tested on several strains, including Top10 and DH5α.

I would like to know if this method has been tested with XL1-blue cells and if using XL1-blue would have a higher transformation efficiency using this method than DH5α.  I am attempting a primer extension mutagenesis based on megaprimer extension (http://www.biotechni...18_O_95037a.pdf), but my transformations haven't produced any colonies yet.  I'd like to prepare highly efficient competent cells that can be used to perform transformations of nicked circular DNA.  Could I achieve this using the CCMB80 method on XL1-blue cells?

[bob1]

I don't see why not, most methods of making competent cells work in a similar fashion, so it should be fine.