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DAPI in mammalian cells


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#1 jay99

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Posted 24 September 2003 - 06:20 AM

I have noted different dapi profiles in the nucleus of mammalian cells. A recurent profile is bright spots over a fainter background (especially seen in liver). Do these spots correlate with heterochromatin because of condensation of DNA ?
I would appreciate any hints regarding this matter,
Thanks

#2 Victor

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Posted 16 October 2003 - 12:09 PM

Yes, but that's not enough to conclude that it's heterochromatin. If it colocalizes with histone antibodies (by fluorescence or immuno EM), then you'll have more substantial evidence.




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