Im just reading the procedure for a biorad (bradford assay) which involves the preparation of a protein blank which is subtracted from all standards and the sample?
Why bother, surely the blankis the same for all of them (unless one has made up a sample in a completely different buffer) so if one reads of the standard curve, one would get the same answer???
Am I wrong?
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#2
mdfenko
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Posted 30 July 2012 - 06:45 AM
you need the blank to zero the spectrophotometer.
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#3
johnuknow
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Posted 30 July 2012 - 06:59 AM
you need the blank to zero the spectrophotometer.
But if I plot the standards without zeroing against then read off the sample tube (again without zeroing).....I will get the same answer as if I had zero. Essentially what zeroing does is subtract the same value from exerything...ie standards, samples etc etc.
But if I plot the standards without zeroing against then read off the sample tube (again without zeroing).....I will get the same answer as if I had zero. Essentially what zeroing does is subtract the same value from exerything...ie standards, samples etc etc.
#4
mdfenko
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Posted 30 July 2012 - 07:26 AM
blanks for the bradford are relatively high absorbance. if you don't zero then you may be taking readings beyond the linear range of the spectrophotometer.
talent does what it can
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i do what i get paid to do
genius does what it must
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